Stress-Inducible Overexpression of SlDDF2 Gene Improves Tolerance against Multiple Abiotic Stresses in Tomato Plant

Dehydration-responsive element-binding protein 1 (DREB1)/C-repeat binding factor (CBF) family plays a key role in plant tolerance against different abiotic stresses. In this study, an orthologous gene of the DWARF AND DELAYED FLOWERING (DDF) members Arabidopsis, SlDDF2, was identified tomato plants. The SlDDF2 expression analyzed, and clear induction response to ABA treatment, cold, salinity, drought stresses observed. Furthermore, two transgenic lines (SlDDF2-IOE#6 SlDDF2-IOE#9) with stress-inducible overexpression under Rd29a promoter were generated. Under stress conditions, significantly higher both lines. growth performance, as well physiological parameters, evaluated wild-type showed retardation phenotypes had chlorophyll content conditions However, relative decrease performance (plant height, leaf number, area) stressed lower than that plants, compared nonstressed conditions. reduction water loss rate also Compared enhanced including deficit, cold. conclusion, can be useful tool improve multiple